| 李树霄,覃艳春,黄志群,卢 柳,林佳媚,苏庆右,张莉莉,林晓群,陆 钢.基于高通量测序技术分析湿润烧伤膏对感染创面菌群特征的影响[J].中国烧伤创疡杂志,2025,(4):253~257. |
| DOI： |
| 中文关键词: 湿润烧伤膏 创面 感染 致病菌 菌群 生物合成 代谢 |
| 英文关键词:Moist exposed burn ointment Wound Infection Pathogenic bacteria Microbiota Biosynthesis Metabolism |
| 基金项目:广西壮族自治区中医药管理局自筹项目 (GXZYZ20210135); 广西自然科学基金联合专项项目 (2025GXNSFHA069054); 国家级大学生创新创业训练计划项目 (202210599006); 广西教育厅大学生创新创业训练计划项目 ( S202310599085, S202310599087) |
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| 中文摘要: |
| 【摘要】 目的 探讨湿润烧伤膏对感染创面菌群种类、特征的影响。方法 选取 2022 年 7 月至 2023 年 6 月右江民族医学院附属医院收治的 5 例应用湿润烧伤膏进行治疗的感染创面患者作为研究对象, 采集治疗前后创面分泌物样本, 将治疗前样本纳入M_SM0组、治疗后样本纳入M_SM3组, 扩增建库及上机信息采集后应用 Illumina 基因测序平台对微生物群落 DNA 片段进行双端测序, 统计物种分类情况, 分析物种组成、丰度、组间差异、α 多样性与功能富集情况。结果 共获得有效序列 1 168 481 条, 物种分类多样, 其中M_SM0组共涉及种类 3 821 个、M_SM3组共涉及种类 3 701 个; 与M_SM0组相比, M_SM3组厚壁菌门、放线菌门、芽单胞菌门、绿弯菌门、互养菌门以及葡萄球菌属、卟啉单胞菌属、嗜胨菌属、肠杆菌属及棒状杆菌属物种数量减少; 与M_SM0组相比, M_SM3组患者创面感染微生物物种丰度发生改变; M_SM0组与M_SM3组患者创面感染微生物差异明显的前 5 个门分类与属分类标志性物种依次为蓝细菌门、变形菌门、厚壁菌门、疣微菌门、放线菌门与肠球菌属、双歧杆菌属、布氏杆菌属、脱硫弧菌属、布劳特氏菌属; M_SM0组与M_SM3组患者创面感染微生物物种 α 多样性无明显差异 (P 均>0.05); M_SM0组和M_SM3组物种基因功能差异主要集中在安莎霉素代谢通路、糖酵解/ 糖异生、丙氨酸与硫等的代谢、硫传递系统、脂肪酸生物合成等, 且每种基因功能均涉及多种物种 (微生物)? 结论 湿润烧伤膏能够通过调节致病菌的生物合成及代谢等抑制其增殖, 改变创面菌群结构。 |
| 英文摘要: |
| 【Abstract】 Objective To study the effects of moist exposed burn ointment (MEBO) on the species composition and microbial characteristics of infected wounds. Methods Five patients with infected wounds treated with MEBO between July 2022 and June 2023 after being admitted to Affiliated Hospital of Youjiang Medical University for Nationalities were enrolled as research subjects. Secretion samples from the wounds were collected before and after treatment. The samples before treatment were included in the M_SM0 group, and those after treatment in the M_SM3 group.After amplification, library construction, and sequencing information collection, the Illumina sequencing platform was used to perform pairedend sequencing of microbial community DNA fragments. Species classification was statistically analyzed, and the composition, abundance, differences, α diversity and functional enrichment were analyzed between the two groups. Results A total of 1,168,481 valid sequences were obtained, with diverse species classification. The M_SM0 group involved 3,821 species, and the M_SM3 group involved 3,701 species. Compared with the M_SM0 group, the M_SM3 group showed a reduction in the number of species in the Firmicutes, Actinobacteria, Gemmatimonadetes, Chloroflexi, Synergistetes, and genera including Staphylococcus, Porphyromonas, Peptoniphilus, Enterobacter, and Corynebacterium. The abundance of wound infection microbial species of patients in the M_SM3 group changed compared with the M_SM0 group. The top 5 phylum and genus classification indicator species with significant differences in wound infection microorganisms between the M_SM0 group and the M_SM3 group were Cyanobacteria, Proteobacteria, Firmicutes, Verrucomicrobia, Actinobacteria,and Enterococcus, Bifidobacterium, Brochothrix, Desulfovibrio, and Blautia, respectively. There was no significant difference in α diversity of wound infection microbial species between the M_SM0 group and the M_SM3 group ( all P>0?? 05). The differences in gene functions between the M_SM0 group and the M_SM3 group mainly focus on the ansamycin biosynthesis pathway, glycolysis/ gluconeogenesis, metabolism of alanine and sulfur compounds, sulfur relay system, and fatty acid biosynthesis, with each gene function involving multiple species (microorganisms). Conclusion MEBO can inhibit the proliferation of pathogenic bacteria by regulating their biosynthesis and metabolism, and alter the wound microbiota composition. |
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